Usnic Acid Activity on Oxidative and Nitrosative Stress of Azole-Resistant Candida albicans Biofilm

MARIANA PERALTA,; MARÍA ANGEL DA SILVA; MARIA GABRIELA ORTEGA; JOSE LUIS CABRERA; MARIA GABRIELA PARAJE

PLANTA MEDICA

GEORG THIEME VERLAG KG

Año: 2017 vol. 83

0032-0943

everal studies report that (+)-usnic acid, a lichen secondary metabolite, inhibits growth of different bacteria and fungi; however, the mechanism of itsantimicrobial activity remains unknown. In this study, we explored the ability of usnic acid, obtained from Usnea amblyoclada, as an antibiofilm agent against azole-resistant and azole-sensitive Candida albicans strains by studying the cellular stress and antioxidant response in biofilms. The biofilm inhibitory concentration of usnic acid (4 µg/mL) exhibited a significant biofilm inhibition, 71.08% for azole-resistant and 87.84% for azole-sensitive C. albicans strains. Confocal scanning laser microscopy showed that the morphologyof mature biofilm was altered (reduced the biomass and thickness) in the presence of usnic acid. The antifungal effect was mediated by an oxidativeand nitrosative stress, with a significant accumulation of intracellular and extracellular reactive oxygen species detected by confocal scanninglaser microscopy and