Trophoblast toxicity of the neonicotinoid insecticide acetamiprid and an acetamiprid-based formulation

GOMEZ, SEBASTIAN DIEGO; BUSTOS, PAMELA SOLEDAD; SÁNCHEZ, VICTORIA GUADALUPE; ORTEGA, MARÍA GABRIELA; GUIÑAZÚ, NATALIA

TOXICOLOGY

ELSEVIER IRELAND LTD

Año: 2020 vol. 431

0300-483X

he neonicotinoid (Neo) insecticide family is a relatively new class of pesticides of growing use. There is an increasing concern that human exposure to environmental pollutants in utero may be associated with diseases in adulthood. A functional placenta and trophoblasts are a requisite for a healthy pregnancy. The aim of this study was to investigate whether the Neo Acetamiprid (Ace) and one of its commercial formulations (Ace CF) display toxic features to a human first trimester trophoblast cell line. HTR-8/SVneo cells were cultured in the presence of Ace or Ace CF (0.1?100 μM) for 4 and 24 h, and changes in cell viability, reactive oxygen species, antioxidant system and macromolecule damage levels were evaluated.Ace and Ace CF are cytotoxic for HTR-8/SVneo trophoblasts. Cell viability loss and oxidative imbalance were triggered by Ace and Ace CF treatments. Impact in the antioxidant enzymes catalase, superoxide dismutase and gluthatione S-transferase activities were observed af