Silver nanoparticles synthesized with quercetin: synthesis, characterization and effect in human trophoblast exposed to neonicotinoid

BUSTOS PAMELA SOLEDAD; LÓPEZ VENDITTI, ELIANA; CABRERA JOSÉ LUIS; ORTEGA MARÍA G.; PAEZ PAULINA; GUIÑAZÚ, NATALIA LORENA

Mar del Plata

Congreso; REUNION CONJUNTA SAIC-SAFE-SAB-SAP 2019; 2019

Sociedad Argentina de Investigación Clínica

Silver nanoparticles gained popularity due to their applications in medicine. The synthesis of nanoparticles using plant extracts or their antioxidant metabolites as reducing and stabilizing agents is the most adopted method for green production. Previous studies showed toxic effects of the insecticide neonicotinoid (Neo) acetamiprid on human trophoblasts mainly due to oxidative imbalance. The aim of this work was to obtain green silver nanoparticles (AgNPs) able to avoid Neo toxicity. AgNPs were synthesized in the presence of the antioxidant plant metabolite quercetin (Q), a flavonoid isolated from Flaveria bidentis.AgNPs synthesis conditions evaluated: AgNO3 (1-10 mM), Q (0.5-2 mM), use of sodium citrate as stabilizer, stirring time (0-24 h) and pH (7-12). AgNPs were characterized by UV/Vis spectroscopy, TEM and DLS. To test the possible protective effect of AgNPs, the HTR-8/SVneo trophoblast cell line was pre-treated 1 h with AgNPs, and then incubated for 24 h with Neo (100 µM). Controls of synthesis precursors, Neo and AgNPs dilutions (1/5-1/1000) were included. Three AgNPs were obtained, AgQ3NPs (18 nm), AgQC4NPs (50 nm) and AgQC7NPs (33 nm). AgQC7NPs, unlike its precursors, showed no cytotoxic effect on trophoblast, while AgQ3NPs and AgQC4NPs caused a significant decrease in cell viability at 1/5 dilution. Regarding the protective AgNPs effect against Neo toxicity, AgQ3NPs and AgQC7NPs at 1/250, 1/500 and 1/1000 dilutions, avoided the decrease of cell viability (MTT method) and the reactive oxygen species (ROS) production (NBT method) induced by Neo. AgQC4NPs, avoided cell viability loss at 1/500 and 1/1000 dilutions, with a concentration-dependent effect in ROS production reaching values significantly similar to the control at 1/1000. Q synthesized green AgNPs demonstrated a protective effect for Neo induced cytotoxicity and oxidative imbalance in trophoblast. Future studies will be necessary to deepen the study of this protective effect.