Reduction of Candida tropicalis biofilm by photoactivation of a Heterophyllaea pustulata extract

MARIONI J; ARCE JE; CABRERA JL; PARAJE MG; NÚÑEZ MONTOYA SC

PHARMACEUTICAL BIOLOGY

TAYLOR & FRANCIS INC

Lugar: Londres; Año: 2016 vol. 3 p. 1 - 1

1388-0209

ontext: Biofilm formation is an important problem, since this growth mode confers resistance to drugs usually used in therapeutics. Objective: In vitro antifungal activity of extracts obtained from Heterophyllaea pustulata Hook f. (Rubiaceae) were studied against Candida tropicalis biofilms, evaluating the effect of irradiation and the oxidative and nitrosative stresses as possible mechanisms of action. Materials and methods: Hexane, benzene, ethyl acetate and ethanol extracts were evaluated at three concentrations (0.2, 0.1 and 0.05mg/mL) over mature biofilm, under darkness and irradiation. After 48 h of incubation, biofilm quantitation was performed by the O?Toole and Kolter method. Reactive oxygen species (ROS) was measured by nitro-blue tetrazolium (NBT) reaction and reactive nitrogen intermediates (RNI) by the Griess reagent. Superoxide dismutase activation (SOD, NBT assay) and total antioxidant system (FRAP test) were studied. Results: Only the benzene extract at 0.2mg/mL reduce