Ultrasonic-assisted extraction of anthraquinones from Heterophyllaea pustulata Hook f. (Rubiaceae) using ethanol-water mixtures

M.F. BARRERA VÁZQUEZ; L.R. COMINI; R.E. MARTINI; S.C. NÚÑEZ MONTOYA; S. BOTTINI; J.L. CABRERA

ELSEVIER SCIENCE BV

Año: 2015 vol. 69 p. 278 - 278

n this study, ultrasound-assisted extraction (UAE) was evaluated as an easier and more efficient alternative method for the isolation of anthraquinones (AQs) from Heterophyllaea pustulata Hook f.(Rubiaceae), compared with conventional extraction techniques. The extraction was performed usingan ethanol-water solution as solvent. The influence of different operating conditions, as temperature,time, solvent composition, and solvent/sample ratio and its relationship with ultrasonic techniques was analyzed. For this purpose and to achieve global optimization of the ultrasound-assisted extraction of H. pustulata, Taguchi experimental design with a L9 orthogonal array was applied.The results showed that the yield obtained with the ultrasound assisted extraction is approximately twice higher than the traditional Soxhlet technique, and Taguchi analysis demonstrated that the most influential factor is solvent concentration, followed by time, temperature, and solvent/sample ratio. The solvent co