Evaluation of the cytotoxicity, genotoxicity and apoptotic induction of an aqueous extract of Achyrocline satureioides (Lam.) DC.

M.C. SABINI; L.N. CARIDDI; F.M. ESCOBAR; F. MAÑAS; L. COMINI; E. REINOSO; S.B. SUTIL; A.C. ACOSTA; S. NÚÑEZ MONTOYA; M.S. CONTIGIANI; S.M. ZANON; L.I. SABINI

PERGAMON-ELSEVIER SCIENCE LTD

Año: 2013 vol. 60 p. 463 - 463

chyrocline satureioides is widely consumed as infusion or aperitif and shows important therapeutic properties. Previously, we reported absence of genotoxicity of cold aqueous extract (CAE) of A. satureioides by Allium test. However, one test cannot predict the genotoxic effects of a substance. Thus, the aim of this work was to investigate cytotoxicity, genotoxicity and apoptotic ability of CAE of A. satureioides. In addition, CAE was chemically characterized. The cytotoxicity was evaluated by Trypan blue and MTT assays. The apoptotic capacity was evaluated by Hoechst staining and DNA fragmentation-analysis. The genotoxicity was studied by comet assay (CA) and micronucleus test. The identification and quantification of flavonoids were performed by HPLC?ESI?MS/MS. The cytotoxicity studies indicated low toxicity of CAE. In addition, CAE did not induce apoptotic effects on human PBMCs. CAE did not show genotoxicity in vitro against Vero cells, at 10?50 lg/mL. CAE did not induce in vivo ge