Inactivation of Herpes Simplex Virus by photosensitizing anthraquinones isolates from Heterophyllaea pustulata

MUGAS M.L.; MARIONI J; MARTINEZ F.; AGUILAR J.J.; CABRERA J.L.; CONTIGIANI MS; KONIGHEIM B.S.; NUÑEZ MONTOYA S.C.

PLANTA MEDICA

GEORG THIEME VERLAG KG

Año: 2021

0032-0943

eterophyllaea pustulata is a phototoxic plant from Argentina. Aerial parts extracts, high in photosensitizing anthraquinones, have shown in vitro antiviral activity. The purpose of this study was to study the antiherpetic activity of the main purified anthraquinones, even evaluating their competence as photodynamic sensitizers to photo-stimulate the antiviral effect. In vitro antiviral activity against Herpes Simplex virus type I and the photo-inactivation of viral particle were studied by the Neutral Red uptake test and observation of the cytopathic effect. Rubiadin 1-methyl ether and 5,5′-bisoranjidiol produced a significant effect (≥ 80% inhibition) with minimal damage to host cells (subtoxic concentration). Anthraquinones with poor antiherpetic activity at its maximum noncytotoxic concentration showed an important photo-stimulated effect, such is the case of soranjidiol and 5,5′-bisoranjidiol (28.0 ± 6.3 vs. 81.8 ± 2.1% and 15.5̴