Enhancement of anthraquinone production and release by combination of culture medium selection and methyl jasmonate elicitation in hairy root cultures of Rubia tinctorum

PERASSOLO, MARÍA; CARDILLO, ALEJANDRA B.; MUGAS, M. LAURA; NÚÑEZ MONTOYA, SUSANA C.; GIULIETTI, ANA M.; RODRÍGUEZ TALOU, JULIÁN

Industrial Crops and Products

ELSEVIER SCIENCE BV

Año: 2017 vol. 105 p. 124 - 124

0926-6690

nthraquinones (AQs) are secondary metabolites widely distributed in nature. Interesting applications of plant extracts containing AQs include the treatment of Hepatitis C and cancer. Plant in vitro culture is an alternative for producing plant-derived pharmaceuticals in controlled conditions and with low environmental impact. Moreover, it allows the application of different strategies for enhancing secondary metabolite production. Hairy root cultures, obtained after Agrobacterium rhizogenes infection, are able to produce high amounts of secondary metabolites at high growth rates. In this work, growth kinetics of hairy root cultures of Rubia tinctorum and AQ production were evaluated in two different culture media, Gamborg B5 with half of the saline strength (B51/2) and Lloyd & Mc Cown´s Woody Plant Medium (WPM). Although WPM allowed higher biomass production (58.6% higher) than B51/2, specific AQ production was higher in B51/2 (between 1.2 and 2.1 fold increases from day 21 to the end