New insights into the antiviral activity of nordihydroguaiaretic acid: Inhibition of dengue virus serotype 1 replication

MARTINEZ, FLORENCIA; GHIETTO, LUCIA MARIA; LINGUA, GIULIANA; MUGAS, M. LAURA; AGUILAR, J. JAVIER; GIL, PEDRO; PISANO, M. BELÉN; MARIONI, JULIANA; PAGLINI, MARÍA GABRIELA; CONTIGIANI, MARTA S.; NÚÑEZ-MONTOYA, SUSANA C.; KONIGHEIM, BRENDA S.

PHYTOMEDICINE

ELSEVIER GMBH

Año: 2022 vol. 106

0944-7113

ackground: Dengue virus (DENV) is considered one of the most important pathogens in the world causing 390 million infections each year. Currently, the development of vaccines against DENV presents some shortcomings and there is no antiviral therapy available for its infection. An important challenge is that both treatments and vaccines must be effective against all four DENV serotypes. Nordihydroguaiaretic acid (NDGA), isolated from Larrea divaricata Cav. (Zygophyllaceae) has shown a significant inhibitory effect on a broad spectrum of viruses, including DENV serotypes 2 and 4. Purpose: We evaluated the in vitro virucidal and antiviral activity of NDGA on DENV serotype 1 (DENV1), including the study of its mechanism of action, to provide more evidence on its antiviral activity. Methods: The viability of viral particles was quantified by the plaque-forming unit reduction method. NDGA effects on DENV1 genome and viral proteins were evaluated by qPCR and immunofluorescence, respectively.