Two new anthraquinones: 1,6-dihydroxy-7-methoxy-2-methylanthraquinone and 2-hydroxy-3-methoxy-6-methylanthraquinone, and a new bianthraquinone: 5,5´-bis (1,6-dihihydroxy-2-methylanthraquinone), were isolated from the leaves of Heterophyllaea pustulata Hook (Rubiáceas). Their structures were established trough spectral means (1H and 13C NMR mono and bidemensional, IR, MS and UV-V).