Photo-inactivation mechanism over Candida biofilms by natural anthraquinones

MARIONI J; BRESOLÍ-OBACH R; PARAJE MG; NONELL S; NÚÑEZ MONTOYA SC

Santiago

Encuentro; 25th INTER-AMERICAN PHOTOCHEMICAL SOCIETY MEETING; 2016

IAPS, Univer. Católica de Chile.

Two photosensitizing anthraquinones (AQs), isolated from Heterophyllaea pustulata Hook f. (Rubiaceae), rubiadin (AQ1) and 1-methyl ether rubiadin (AQ2), showed antifungal activity on Candida tropicalis biofilms under actinic irradiation. It was hypothesized that the photoinactivation mechanism involves the Reactive Oxygen Species (ROS): superoxide anion (O2?-) and/or singlet molecular oxygen (1O2). In this work, we set out to identify specifically the reactive intermediates involved.