Effect of irradiation frequency in photodynamic inactivation of Candida tropicalis biofilms by natural anthraquinones in combination with antifungals

MARIONI J; CABRERA JL; PARAJE MG; NUÑEZ MONTOYA S.C.

Mar del Plata

Otro; Medicina - XLVIII REUNIÓN ANUAL DE SAFE; 2016

Sociedad Argentina de Farmacología Experimental

A new strategy to improve the effect of antifungal agents against biofilms is their combination with natural compounds. We proved that rubiadin (1) and rubiadin 1-methyl ether (2), two natural photosensitizing anthraquinones (AQs), reduce the biofilm formation of C. tropicalis due to a photodynamic action. Antibiofilm effect of each AQ was enhanced by applying an irradiation sequence. Thus, the bioactive concentration of each AQ was halved, achieving also an increase in the reducing effect only in the case of 2 (63 ± 7 %R).The aim of this work was to expand the antibiofilm activity of these AQs against C. tropicalis, applying the same irradiation sequence but using each AQ combined each other or with amphotericin B (AmB).