Construction of layer-by-layer self-assemblies of glucose oxidase and cationic polyelectrolyte onto glassy carbon electrodes and electrochemical study of the redox-mediated enzymatic activity

NANCY FERREYRA, LILIANE COCHE-GUÉRENTE,PIERRE LABBÉ

ELECTROCHIMICA ACTA

Año: 2004 vol. 49 p. 477 - 477

0013-4686

font face="Times New Roman" size="1"> Build-up of enzyme?polyelectrolyte multilayer onto glassy carbon (GC) surfaces by electrostatic self-assembling method has been investigated.In order to functionalize GC surface by a starting negatively charged layer, two approaches have been carried out: (i) covalent inkageof phenyl acetic acid through electroreduction of 4-phenylacetic diazonium salt (GCA surface), and (ii) formation of a glucose oxidase (GOD) monolayer through an affinity reaction between a GOD conjugated antibody and a previously adsorbed antigen monolayer (GCB surface).GCA and GCB surfaces have been modified by a