Electrochemical behaviour of Nitrate Reductase immobilized in self-assembled structures with redox poliviologen

N. F. FERREYRA, L.GUERENTE, P.LABBÉ, V.SOLIS, E.CALVO

LANGMUIR

Año: 2003 vol. 19 p. 3864 - 3864

0743-7463

font size="1" face="Times New Roman"> We report on a novel bioelectrode based on self-assembled multilayers of nitrate reductase (NR) intercalated with a cationic viologen-functionalized polyvinylpyridinium (PV) polymer specially synthesized for this purpose. Different samples of polymer proved to have varying substitution ratios, according to NMR experiments. The electrostatic self-assemblies were built up on a thiol-modified gold surface using a strategy previously described by Hodak et al. The electrochemical behavior of PV and the response of the electrodes toward nitrate were followed by cyclic voltammetry. The catalytic currents obtained were proportional to the number of NR-immobilized layers, as well as to the polymer substitution degree, proving that the enzyme remained active