Voltammetry of Osmium End-Labeled Oligodeoxynucleotides at Carbon, Mercury, and Gold Electrodes

MOJMÍR TREFULKA , NANCY FERREYRA , VERONIKA OSTATNÁ, MIROSLAV FOJTA , GUSTAVO RIVAS , EMIL PALEEK

WILEY-V C H VERLAG GMBH

Año: 2007 vol. 19 p. 1334 - 1334

Voltammetric behavior of oligodeoxynucleotide (ODN) 5-T40 (GAA)7-3 end-labeled with osmium tetroxide,2,2-bipyridine [Os(VIII)bipy] was compared with Os(VIII)bipy-base- and with Os(VI)bipy-sugar-modified thymine ribosides. Cyclic voltammograms of Os(VIII)bipy-modified ODN at mercury and carbon electrodes were similar but not identical to those of Os(VIII)bipy-modified thymine riboside. Treatment of the ODN with Os(VI)bipy did not result in the ODN modification, in agreement with the known specificity of the reagent to the sugar cis-diols. We show that in addition to mercury and carbon electrodes, the gold electrode can be used to detec