Asparagine Quantification in cellular culture media using copper modified carbon nanotubes composite Electrodes

MARIA LORENA LÓPEZ RODRIGUEZA, GUILLERMINA L. LUQUE, CARMELO J. FELICEB, NANCY F. FERREYRA, ROSSANA E. MADRID, GUSTAVO A. RIVAS, CARLA E. GIACOMELLI; GUILLERMINA L. LUQUE; ROSSANA E. MADRID, CARMELO J. FELICE; NANCY F FERREYRA; CARLA GIACOMELLI; GUSTAVO A. RIVAS

ELSEVIER SCIENCE SA

Año: 2011 vol. 158 p. 423 - 423

p style="margin: 0in 0in 0pt; text-align: justify; line-height: 150%; text-indent: 18.45pt;" class="MsoNormal">Direct quantification of Asparagine (Asp) in a culture medium containing Streptomyces sp. M7 was performed using multi-wall carbon nanotubes composite electrodes containing copper microparticles. Independently of the culture medium, sensitive, well-defined and fast (steady-state signal reached within 10s) responses are obtained at CNTPE-Cu after additions of Asp. No interference was observed due to the culture medium components. The high sensitivity of the method made possible to determine the decrease of Asp concentration as a function of the bacteria growth. The results reveal that the SM7 bacterium can consume Asp as a carbon and nitrogen sources in the absence of another carbon source such as glucose.