Differential tissue and flight developmental expression of glycerol-3-phosphate dehydrogenase isozymes in chagas disease vector

STROPPA, M; CARRIAZO, C; SORIA, N; PEREIRA, R; GEREZ DE BURGOS, N

High Wire Press

Lugar: Stanford; Año: 2008 vol. 79 p. 28 - 28

p style="margin: 0cm 0cm 0pt -0.75pt; text-indent: 14.95pt; line-height: 200%; text-align: justify;" class="MsoNormal">Glycerol-3-phosphate dehydrogenase (GPDH) isozymes are differentially expressed among tissues and during flight development. We have isolated and characterized from Triatoma infestans (T. infestans), Chagas´ disease vector, two different cDNA encoding for GPDH-1 and GPDH-2 isozymes. After sequencing complete cDNAs, the inferred amino acid sequences showed high identity (60-70 %) with others GPDH sequences from flying insects.  The amino acid residues responsible for the catalytic activity and NAD+ binding sites are essentially conserved.  GPDH-1 is involved in the flight-muscle metabolism and GPDH-2 provides in many tissues precursors for lipid biosynthesis.  In fifth instar nymphs thoracic muscle