Congresos y reuniones científicas
Título:
EXPRESSION OF GPDH ISOFORMS IN FLIGHT MUSCLE FROM WILD AND LABORATORY Triatoma infestans ADULTS
Autor/es:
STROPPA MM, CARRIAZO C, LAGUNAS M, REYNOSO R, PEREIRA R, GEREZ DE BURGOS NM.
Reunión:
Congreso; XLVI Reunión Anual Sociedad Argentina de Investigación Bioquímica y Biología Molecular; 2010
Resumen:
Triatoma
infestans (T.
infestans), vector of Chagas disease, acquires wings and develops flight
ability after shedding the last nymphal stage to adult. The flight is important
in the recolonization of the housing post fumigation. The enzyme
glycerol-3-phosphate dehydrogenase (GPDH) is involved in providing energy for
flight. In T. infestans, we characterized two isoforms of GPDH
transcripts (GPDH-1 and GPDH-2). Previous studies showed that its expression in
flight muscle varies during the development of flight ability, according to sex
and to changes in temperature and time of intake. The aim of this study was to
analyze the expression of the isoforms of GPDH in flight muscle of T.
infestans adult from natural population and laboratory colonies. We were
performed semiquantitative RT-PCR, non-denaturing PAGE electrophoresis revealed
with specific enzyme activity and determination of enzymatic total activity. We
found that GPDH-1 is the predominant isoform in flight muscle T. infestans
adult natural population and laboratory colonies. In laboratory colonies, comparing
the expression in the first with the second generation, there are an increase
in the expression of GPDH-2 and a decrease in the expression of GPDH-1. This is
consistent with the metabolic functions described for isoforms of GPDH and the
adjustment to a regular supply of food.