. Use of anAmino-cupric-silver technique for the detection of early and semiacute neuronal Degeneration caused by neurotoxicants, hypoxia, and physical trauma

DE OLMOS JS, BELTRAMINO CA, DE OLMOS DE LORENZO S

PERGAMON-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 1994 vol. 16 p. 545 - 545

p>A new amino-cupric silver protocol is described for detection of neuronal degeneration. We describe its selectivity in visualizing both early and semiacute degeneration after intracerebral or systemic administration of a variety of neurotoxicants in rats, and after transient ischemic episodes in gerbils. As early as 5 min after physical trauma, or 15 min following either intrastriatal injections of glutamate analogs or exposure to ischemic episodes, neuronal silver staining was evident at primary sites of trauma (i.g. injection sites) and at hodologically related secondary sites. With intoxication by peripheral injections of trimethyltin (IP) or intracerebral injections of Doxorubicin, reproducible patterns of degeneration are demonstrable after 24 h or after 9-13 days, respectively. The amino-cupric silver method permits simultaneous detection of all neuronal compartments against a clear background. Degeneration in the neuronal cell bodies, dendrites, axons and terminals, as well a