LIPID PROFILING OF BARLEY ROOT IN INTERACTION WITH FUSARIUM MACROCONIDIA

REYNA, MATIAS; PEPPINO MARGUTTI, MICAELA; VILLASUSO, ANA LAURA

ENVIRONMENTAL AND EXPERIMENTAL BOTANY

PERGAMON-ELSEVIER SCIENCE LTD

Año: 2019

0098-8472

usarium is the major causal agent of Fusarium head blight (FHB) in several cereal crops. Macroconidia, the asexual form of Fusarium, can infect the roots and aerial parts of plants. The response of cereal to attacks by Fusarium has been investigated at transcriptomic and metabolomic level, but there are no reports of root lipidomic assays carried out during Fusarium-root interaction. In order to determine how root phospholipids contribute to that interaction, we performed a lipidomics-based ESI-MS/MS assay coupled with a statistical analysis. It was found that phospholipid and galactolipid levels were not modified during early pathogen response, although some individual phospholipid molecular species did undergo significant changes. In plants exposed to macroconidia there was a rapid and transient increase in the phosphatidylcholine (36:4-PC) molecular species, in comparison with the control. By contrast, there was a decrease in lysophosphatidylcholine (16:0-, 18:2- and 18:3-LPC) leve