Membrane Rigidity and Phosphatidic Acid (PtdOH) Signal: Two Important Events in Acinetobacter guillouiae SFC 500-1A Exposed to Chromium(VI) and Phenol

FERNANDEZ, M; PAULUCCI, N; PEPPINO MARGUTTI, M.; BIASUTTI, A; RACAGNI, G.; VILLASUSO, AL.; AGOSTINI, E; GONZALEZ, P

LIPIDS

SPRINGER HEIDELBERG

Lugar: HEIDELBERG; Año: 2019

0024-4201

The remodeling of membrane lipids is a mechanism that allows microorganisms to survive in unfavorableenvironments such as industrial effluents, which often contain inorganic and organic pollutants, like chromium andphenol. In the present work, we evaluated the effect ofCr(VI) and phenol on the membrane of Acinetobacterguillouiae SFC 500-1A, a bacterial strain isolated from tannery sediments where such pollutants can be found. Thepresence of lipid kinases and phospholipases and thechanges in their activities under exposure to these pollutants were determined. Cr(VI) and Cr(VI) + phenol causedthe membrane to become more rigid for up to 16 h afterexposure. This could be due to an increase in cardiolipin(Ptd2Gro) and a decrease in phosphatidylethanolamine(PtdEtn), which are indicative of more order and rigidity inthe membrane. Increased phospholipase A activity (PLA,EC 3.1.1.4) could be responsible for the decrease in PtdEtnlevels. Moreover, our results indicate that Cr(VI) andCr(VI) + p