PHOSPHOLIPIDS SIGNALLING AND PROLINE ACUMULATION DURING COLD RESPONSE IN Hordeum vulgare

PEPPINO MARGUTTI, M.; REYNA, M.; RACAGNI, G.; VILLASUSO, A.

San Miguel de Tucumán

Congreso; XVII Annual Meeting of the Argentine Biology Society; 2015

Universidad Nacional de Tucumán

PHOSPHOLIPIDS SIGNALLING AND PROLINE ACUMULATION DURING COLD RESPONSE IN Hordeum vulgare Peppino Margutti, M., Reyna, M., Racagni, G., Villasuso, A. Dpto. Biol. Molecular. UNRC. Río Cuarto (Cba). E-mail: mpeppino@exa.unrc.edu.arLow temperature is perceived by plasma membrane as affecting its fluidity and the acumulation of compatible osmolytes. Lipid signalling is regulated by the activities of phospholipases (PLC-PLD), phosphatases (LPPs) and lipid kinases (DGK-PAK) through the dinamic changes in signal lipids as PA and DGPP. The aim of this study was to determine the lipid kinase activities in relation with proline accumulation during the cold exposition. Seedling grown at 25ºC for 4d were allowed at 4ºC for 30-180 min. Cold seedling exposition by 180 min evoked a increase on PAK and DGK activities in root tissue. In contrast, PA and DGPP levels were not modified by cold treatment in young leaves. Cold seedling exposition triggered the proline accumulation in young leaves while there were no significant changes observed on root tissues. This suggested a possible translocation of proline from the root to the young leaves. Proline accumulated because the balance between its synthesis and its catabolism was altered or because the proline was traslocated. To test this hypothesis, we have impaired its movement through the use of physical methods. In relation to this, under this condition, no proline accumulation was observed in young leaves. The results obtained allow us to suggest that the changes in the lipid kinase activities with its corresponding levels of PA and DGPP could be modulating the proline metabolism and its translocation to compensate the effect provoked by low temperatures in Hordeum vulgare.