Differential acyl-editing of PA involves theup-regulation of PC-remodelling genes during chilling recovery in barley

VILCHEZ, AC.; PEPPINO MARGUTTI, M.; REYNA, M; VILLASUSO, AL.; WILKE, NATALIA

Santa Fe (online)

Congreso; Reunión Argentina de Fisiologia Vegetal; 2021

Regulation of the level of saturation and the constitution of glycerolipids is important for plants to maintain the integrity and fluidity of their membranes under temperature stresses and when they are released. A mass spectrometry analysis was performed to compare the lipid profile in barley roots both under chilling stress treatment and in the subsequent recovery to stress. The results indicate that lipid metabolism was significantly affected by chilling. Most of the glycerolipids analysed returned to control values during short-and long-term recovery, whereas several representative phosphatidic acid (PA) molecular species were edited during long-term recovery. Most of the PA molecular species that increased in the long-term had the same acyl chains as the phosphatidylcholine (PC) species that decreased including C34:2 and C36:4 as remarkable changes. To understand the mechanisms underlying the acyl-editing of PC in barley roots, we evaluated the contribution of lysophosphatidylcholine acyltransferases (HvLPCAT) and phospholipase A (HvPLA). In line with the a forementioned results, the expression of the HvLPCAT and HvPLA genes was up-regulated during recovery from chilling. Thus,results showed a differential acyl-editing of PA during recovery, which involves the up-regulation ofPC -remodelling genes.Additional biochemical and biophysical analyses will help us to unravel how these recoveryformed PA -polyunsaturated species modulate themembrane configuration and properties for further contribute withthe knowledge ofcold tolerance in barley.