Surviving nebulization-induced stress: dexamethasone in pH-sensitive archaeosomes

MARIA JULIA ALTUBE; SOLANGE MAILEN SELZER

NANOMEDICINE

FUTURE MEDICINE LTD

Lugar: Londres; Año: 2016

1743-5889

ims. To increase the subcellular delivery of dexamethasone phosphate (DP) and stability to nebulizationstress, pH-sensitive nanoliposomes exhibiting archaeolipids acting as ligands for scavenger receptors(pH-sensitive archaeosomes (ApH) were prepared.Materials & methods. The anti-inflammatory effect of 0.18 mg DP/mg total lipid, 100-150 nm DPcontainingApH (dioleylphosphatidylethanolamine (DOPE): Halorubrum tebenquichense total polararchaeolipids (TPA): cholesterylhemisuccinate (CHEMS) 4.2:2.8:3 w:w) was tested on different cell lines.Size and HPTS retention of ApH and conventional pH-sensitive nanoliposomes (LpH, DOPE:CHEMS 7:3w:w) before and after nebulization were determinedResults & conclusions. DP-ApH suppressed IL-6 and TNF-α on phagocytic cells. Nebulized after 6-month storage, LpH increased size and completely lost its HPTS while ApH3 conserved size andpolydispersity, fully retaining its original HPTS content