Immobilization of Concanavalin A on iron oxide magnetic nanoparticles. Effect of Bovine Serum Albumin in the recognition interactions of the lectin

SELZER, SOLANGE M.

Surfaces and interfaces

Elsevier

Lugar: Amsterdam; Año: 2022 vol. 30

2468-0230

ron oxide magnetic nanoparticles are engaging platforms for diverse nanobiotechnological applications, such as proteins separation and glico-biosensing mediated by the surface functionalization with lectins as recognition elements. We present a new strategy for the union of the lectin Concanavalin A (ConA) at the surface of carboxylated iron oxide magnetic nanoparticles (IOMNPs-Ar-COOH). The nanoparticles were obtained by a single step synthesis in the presence of the aryl diazonium salt derived from 4-aminobenzoic acid. The spontaneous grafting allowed to obtain carboxylic acid covalently functionalized magnetite nanoparticles with the carboxylate group exposed to the solution, as was evidenced through the exhaustive characterization by XRD, FTIR, Raman spectroscopy, and TEM analysis. The nanoparticles of Fe3O4 of (6.6 ± 0.7) nm diameter exhibited good magnetic field responsiveness towards an external magnet and stability towards the environmental oxidation. The carboxylic acid group