Trypanosoma cruzi: transfer of protection by lymph node cells obtained from mice immunized with exoantigens of pI 4.5.

GRUPPI A, CERBAN F, PISTORESI-PALENCIA MC, VOTTERO-CIMA E.

EXPERIMENTAL PARASITOLOGY

ACADEMIC PRESS INC ELSEVIER SCIENCE

Lugar: Amsterdam; Año: 1995 vol. 80 p. 382 - 382

0014-4894

xoantigens of pI 4.5 (Ea 4.5) of T. cruzi released to the circulation of infected mice are able to induce partial protective immune response in mice (F. Cerbán et al. 1991, International Archives of Allergy and Applied Immunology 96, 35-40). In order to analyze the participation of cellular immunity in the parasitemia control, we i.d. immunized mice with Ea 4.5 plus Bordetella pertussis as adjuvant. The role of immune cells in protective immunity was examined by adoptive transfer experiments. The immune lymph node cells (LNC) transferred the capacity to control the parasitemia, since it was observed that the normal recipients of immune LNC, which were afterward infected, presented a significant decrease in parasite levels with respect to the animals receiving LNC from control mice. This capacity was absent in the spleen cells. In addition, polystyrene nonadherent cells from immune LNC transferred the capacity to control T. cruzi infection. It was observed that Ig+ cells and enriched T