Fasciola hepatica Kunitz type molecule decreases dendritic cell activation and their ability to induce inflammatory responses

FALCON CR; MASIH DT; GATTI G; SANCHEZ MC; MOTRAN CC; CERVI L

PUBLIC LIBRARY SCIENCE

Lugar: San Francisco; Año: 2014 vol. 8

he complete repertoire of proteins with immunomodulatory activity in Fasciola hepatica (Fh) has not yet been fully described. Here, we demonstrated that Fh total extract (TE) reduced LPS-induced DC maturation, and the DC ability to induce allogeneic responses. After TE fractionating, a fraction lower than 10 kDa (F ,10 kDa) was able to maintain the TE properties to modulate the DC pro- and anti-inflammatory cytokine production induced by LPS. In addition, TE or F ,10 kDa treatment decreased the ability of immature DC to stimulate the allogeneic responses and induced a novo allogeneic CD4 +CD25 +Foxp3 + T cells. In contrast, treatment of DC with T/L or F ,10 kDa plus LPS (F ,10/L) induced a regulatory IL- 27 dependent mechanism that diminished the proliferative and Th1 and Th17 allogeneic responses. Finally, we showed that a Kunitz type molecule (Fh-KTM), present in F ,10 kDa, was responsible for suppressing pro-inflammatory cytokine production in LPS-activated DC, by print