13th International Congress of Immunology, Rio de Janeiro, Brazil, Aug 21-25, 2007
th International Congress of Immunology, Rio de Janeiro, Brazil, Aug 21-25, 2007P0939
Excretory secretory products (ESP) from a helminth parasite induce apoptosis and
confer anti-inflammatory properties to myeloid dendritic cells.
L. A. Cervi, C. R. Falcon, C. C. Motran;
Faculty of Chemical sciences, Cordoba, Argentina.
Faculty of Chemical sciences, Cordoba, Argentina.
Faculty of Chemical sciences, Cordoba, Argentina.
, C. R. Falcon, C. C. Motran;Faculty of Chemical sciences, Cordoba, Argentina.
, C. R. Falcon, C. C. Motran;Faculty of Chemical sciences, Cordoba, Argentina.
In infectious diseases, dendritic cells (DCs) are able to sense pathogens, integrate this
information and regulate the quantity and quality of adaptive immune response. During its
migration through the host, the helminth parasite Fasciola hepatica release different products
which contact with immune cells, among which are DCs. In this work, we study the ability of ESP to modulate the activation of TLR-stimulated DCs and their effect on the induction of allogeneic T cell responses. ESP affect the capacity of LPS-treated DCs to up-regulate the expression of CD40, CD80, CD86 being the highest effect on CD40 (from 56% to 7%) In addition, IL-12 and TNF�� production were also diminished in ESP-LPS- treated DCs. ESP were
also able to induce apoptosis of immature or LPS-mature DCs. CD40 down-regulation observed in ESP-LPS treated DCs, was not related to the diminished viability of DCs, since annexin-V (-), 7-AAD (-) DCs, also showed a marked diminution in this molecule. Exposure of DCs to ESP impairs the ability of immature or LPS-activated DCs to prime alloantigen specific T cell response and INF�� production in vivo and in vitro. Our findings suggest, that ESP-DCs contact
impairs the viability and function of these cells. This fact could be a strategy of the parasite to prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP could be exploited as a novel approach for down-regulating unwanted T cell response. impairs the viability and function of these cells. This fact could be a strategy of the parasite to prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP could be exploited as a novel approach for down-regulating unwanted T cell response. impairs the viability and function of these cells. This fact could be a strategy of the parasite to prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP could be exploited as a novel approach for down-regulating unwanted T cell response. also able to induce apoptosis of immature or LPS-mature DCs. CD40 down-regulation observed in ESP-LPS treated DCs, was not related to the diminished viability of DCs, since annexin-V (-), 7-AAD (-) DCs, also showed a marked diminution in this molecule. Exposure of DCs to ESP impairs the ability of immature or LPS-activated DCs to prime alloantigen specific T cell response and INF�� production in vivo and in vitro. Our findings suggest, that ESP-DCs contact
impairs the viability and function of these cells. This fact could be a strategy of the parasite to prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP could be exploited as a novel approach for down-regulating unwanted T cell response. impairs the viability and function of these cells. This fact could be a strategy of the parasite to prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP could be exploited as a novel approach for down-regulating unwanted T cell response. impairs the viability and function of these cells. This fact could be a strategy of the parasite to prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP could be exploited as a novel approach for down-regulating unwanted T cell response. also able to induce apoptosis of immature or LPS-mature DCs. CD40 down-regulation observed in ESP-LPS treated DCs, was not related to the diminished viability of DCs, since annexin-V (-), 7-AAD (-) DCs, also showed a marked diminution in this molecule. Exposure of DCs to ESP impairs the ability of immature or LPS-activated DCs to prime alloantigen specific T cell response and INF�� production in vivo and in vitro. Our findings suggest, that ESP-DCs contact
impairs the viability and function of these cells. This fact could be a strategy of the parasite to prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP could be exploited as a novel approach for down-regulating unwanted T cell response. impairs the viability and function of these cells. This fact could be a strategy of the parasite to prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP could be exploited as a novel approach for down-regulating unwanted T cell response. impairs the viability and function of these cells. This fact could be a strategy of the parasite to prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP could be exploited as a novel approach for down-regulating unwanted T cell response.
which contact with immune cells, among which are DCs. In this work, we study the ability of
ESP to modulate the activation of TLR-stimulated DCs and their effect on the induction of
allogeneic T cell responses. ESP affect the capacity of LPS-treated DCs to up-regulate the
expression of CD40, CD80, CD86 being the highest effect on CD40 (from 56% to 7%) In
addition, IL-12 and TNF�� production were also diminished in ESP-LPS- treated DCs. ESP were
also able to induce apoptosis of immature or LPS-mature DCs. CD40 down-regulation observed in ESP-LPS treated DCs, was not related to the diminished viability of DCs, since annexin-V (-), 7-AAD (-) DCs, also showed a marked diminution in this molecule. Exposure of DCs to ESP impairs the ability of immature or LPS-activated DCs to prime alloantigen specific T cell response and INF�� production in vivo and in vitro. Our findings suggest, that ESP-DCs contact
impairs the viability and function of these cells. This fact could be a strategy of the parasite to prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP could be exploited as a novel approach for down-regulating unwanted T cell response. impairs the viability and function of these cells. This fact could be a strategy of the parasite to prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP could be exploited as a novel approach for down-regulating unwanted T cell response. impairs the viability and function of these cells. This fact could be a strategy of the parasite to prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP could be exploited as a novel approach for down-regulating unwanted T cell response.
also able to induce apoptosis of immature or LPS-mature DCs. CD40 down-regulation observed
in ESP-LPS treated DCs, was not related to the diminished viability of DCs, since annexin-V (-),
7-AAD (-) DCs, also showed a marked diminution in this molecule. Exposure of DCs to ESP
impairs the ability of immature or LPS-activated DCs to prime alloantigen specific T cell
response and INF�� production in vivo and in vitro. Our findings suggest, that ESP-DCs contact
impairs the viability and function of these cells. This fact could be a strategy of the parasite to prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP could be exploited as a novel approach for down-regulating unwanted T cell response.
impairs the viability and function of these cells. This fact could be a strategy of the parasite to
prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP
could be exploited as a novel approach for down-regulating unwanted T cell response.
impairs the viability and function of these cells. This fact could be a strategy of the parasite to
prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP
could be exploited as a novel approach for down-regulating unwanted T cell response.
also able to induce apoptosis of immature or LPS-mature DCs. CD40 down-regulation observed
in ESP-LPS treated DCs, was not related to the diminished viability of DCs, since annexin-V (-),
7-AAD (-) DCs, also showed a marked diminution in this molecule. Exposure of DCs to ESP
impairs the ability of immature or LPS-activated DCs to prime alloantigen specific T cell
response and INF�� production in vivo and in vitro. Our findings suggest, that ESP-DCs contact
impairs the viability and function of these cells. This fact could be a strategy of the parasite to prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP could be exploited as a novel approach for down-regulating unwanted T cell response.
impairs the viability and function of these cells. This fact could be a strategy of the parasite to
prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP
could be exploited as a novel approach for down-regulating unwanted T cell response.
impairs the viability and function of these cells. This fact could be a strategy of the parasite to
prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP
could be exploited as a novel approach for down-regulating unwanted T cell response.
which contact with immune cells, among which are DCs. In this work, we study the ability of
ESP to modulate the activation of TLR-stimulated DCs and their effect on the induction of
allogeneic T cell responses. ESP affect the capacity of LPS-treated DCs to up-regulate the
expression of CD40, CD80, CD86 being the highest effect on CD40 (from 56% to 7%) In
addition, IL-12 and TNF�� production were also diminished in ESP-LPS- treated DCs. ESP were
also able to induce apoptosis of immature or LPS-mature DCs. CD40 down-regulation observed in ESP-LPS treated DCs, was not related to the diminished viability of DCs, since annexin-V (-), 7-AAD (-) DCs, also showed a marked diminution in this molecule. Exposure of DCs to ESP impairs the ability of immature or LPS-activated DCs to prime alloantigen specific T cell response and INF�� production in vivo and in vitro. Our findings suggest, that ESP-DCs contact
impairs the viability and function of these cells. This fact could be a strategy of the parasite to prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP could be exploited as a novel approach for down-regulating unwanted T cell response. impairs the viability and function of these cells. This fact could be a strategy of the parasite to prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP could be exploited as a novel approach for down-regulating unwanted T cell response. impairs the viability and function of these cells. This fact could be a strategy of the parasite to prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP could be exploited as a novel approach for down-regulating unwanted T cell response.
also able to induce apoptosis of immature or LPS-mature DCs. CD40 down-regulation observed
in ESP-LPS treated DCs, was not related to the diminished viability of DCs, since annexin-V (-),
7-AAD (-) DCs, also showed a marked diminution in this molecule. Exposure of DCs to ESP
impairs the ability of immature or LPS-activated DCs to prime alloantigen specific T cell
response and INF�� production in vivo and in vitro. Our findings suggest, that ESP-DCs contact
impairs the viability and function of these cells. This fact could be a strategy of the parasite to prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP could be exploited as a novel approach for down-regulating unwanted T cell response.
impairs the viability and function of these cells. This fact could be a strategy of the parasite to
prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP
could be exploited as a novel approach for down-regulating unwanted T cell response.
impairs the viability and function of these cells. This fact could be a strategy of the parasite to
prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP
could be exploited as a novel approach for down-regulating unwanted T cell response.
also able to induce apoptosis of immature or LPS-mature DCs. CD40 down-regulation observed
in ESP-LPS treated DCs, was not related to the diminished viability of DCs, since annexin-V (-),
7-AAD (-) DCs, also showed a marked diminution in this molecule. Exposure of DCs to ESP
impairs the ability of immature or LPS-activated DCs to prime alloantigen specific T cell
response and INF�� production in vivo and in vitro. Our findings suggest, that ESP-DCs contact
impairs the viability and function of these cells. This fact could be a strategy of the parasite to prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP could be exploited as a novel approach for down-regulating unwanted T cell response.
impairs the viability and function of these cells. This fact could be a strategy of the parasite to
prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP
could be exploited as a novel approach for down-regulating unwanted T cell response.
impairs the viability and function of these cells. This fact could be a strategy of the parasite to
prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP
could be exploited as a novel approach for down-regulating unwanted T cell response.
Fasciola hepatica release different productswhich contact with immune cells, among which are DCs. In this work, we study the ability of
ESP to modulate the activation of TLR-stimulated DCs and their effect on the induction of
allogeneic T cell responses. ESP affect the capacity of LPS-treated DCs to up-regulate the
expression of CD40, CD80, CD86 being the highest effect on CD40 (from 56% to 7%) In
addition, IL-12 and TNF�� production were also diminished in ESP-LPS- treated DCs. ESP were
also able to induce apoptosis of immature or LPS-mature DCs. CD40 down-regulation observed in ESP-LPS treated DCs, was not related to the diminished viability of DCs, since annexin-V (-), 7-AAD (-) DCs, also showed a marked diminution in this molecule. Exposure of DCs to ESP impairs the ability of immature or LPS-activated DCs to prime alloantigen specific T cell response and INF�� production in vivo and in vitro. Our findings suggest, that ESP-DCs contact
impairs the viability and function of these cells. This fact could be a strategy of the parasite to prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP could be exploited as a novel approach for down-regulating unwanted T cell response. impairs the viability and function of these cells. This fact could be a strategy of the parasite to prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP could be exploited as a novel approach for down-regulating unwanted T cell response. impairs the viability and function of these cells. This fact could be a strategy of the parasite to prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP could be exploited as a novel approach for down-regulating unwanted T cell response.
also able to induce apoptosis of immature or LPS-mature DCs. CD40 down-regulation observed
in ESP-LPS treated DCs, was not related to the diminished viability of DCs, since annexin-V (-),
7-AAD (-) DCs, also showed a marked diminution in this molecule. Exposure of DCs to ESP
impairs the ability of immature or LPS-activated DCs to prime alloantigen specific T cell
response and INF�� production in vivo and in vitro. Our findings suggest, that ESP-DCs contact
impairs the viability and function of these cells. This fact could be a strategy of the parasite to prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP could be exploited as a novel approach for down-regulating unwanted T cell response.
impairs the viability and function of these cells. This fact could be a strategy of the parasite to
prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP
could be exploited as a novel approach for down-regulating unwanted T cell response.
impairs the viability and function of these cells. This fact could be a strategy of the parasite to
prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP
could be exploited as a novel approach for down-regulating unwanted T cell response.
also able to induce apoptosis of immature or LPS-mature DCs. CD40 down-regulation observed
in ESP-LPS treated DCs, was not related to the diminished viability of DCs, since annexin-V (-),
7-AAD (-) DCs, also showed a marked diminution in this molecule. Exposure of DCs to ESP
impairs the ability of immature or LPS-activated DCs to prime alloantigen specific T cell
response and INF�� production in vivo and in vitro. Our findings suggest, that ESP-DCs contact
impairs the viability and function of these cells. This fact could be a strategy of the parasite to prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP could be exploited as a novel approach for down-regulating unwanted T cell response.
impairs the viability and function of these cells. This fact could be a strategy of the parasite to
prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP
could be exploited as a novel approach for down-regulating unwanted T cell response.
impairs the viability and function of these cells. This fact could be a strategy of the parasite to
prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP
could be exploited as a novel approach for down-regulating unwanted T cell response.
�� production were also diminished in ESP-LPS- treated DCs. ESP werealso able to induce apoptosis of immature or LPS-mature DCs. CD40 down-regulation observed
in ESP-LPS treated DCs, was not related to the diminished viability of DCs, since annexin-V (-),
7-AAD (-) DCs, also showed a marked diminution in this molecule. Exposure of DCs to ESP
impairs the ability of immature or LPS-activated DCs to prime alloantigen specific T cell
response and INF�� production in vivo and in vitro. Our findings suggest, that ESP-DCs contact
impairs the viability and function of these cells. This fact could be a strategy of the parasite to prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP could be exploited as a novel approach for down-regulating unwanted T cell response.
impairs the viability and function of these cells. This fact could be a strategy of the parasite to
prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP
could be exploited as a novel approach for down-regulating unwanted T cell response.
impairs the viability and function of these cells. This fact could be a strategy of the parasite to
prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP
could be exploited as a novel approach for down-regulating unwanted T cell response.
�� production in vivo and in vitro. Our findings suggest, that ESP-DCs contactimpairs the viability and function of these cells. This fact could be a strategy of the parasite to
prevent the developing of an effector T cells response. Besides, the treatment of DCs with ESP
could be exploited as a novel approach for down-regulating unwanted T cell response.
