Cathepsin L3 from Fasciola hepatica induces IFN-γ response in CD4 and CD8 T cells.

CELIAS D; CORVO I; TORT J; SILVANE L; MOTRAN,.; CLAUDIA C; . CERVI L.

Bs As

Congreso; IV LASID Meeting. LXIII Argentinean Immunology Society Meeting. II French-Argentinean Immunology Meeting; 2015

LASID-SAI-FAIC

Cathepsin L3 from Fasciola hepatica induces IFN-γ response in CD4 and CD8T cells Daiana Celias1, Ileana Corvo2, José Tort2, Leonardo Silvane1, Cristina Motrán1 y Laura Cervi1dcelias@fcq.unc.edu.ar; icorvo@fmed.edu.uy ; jtort@fmed.edu.uy; lsilvane@fcq.unc.edu.ar; cmotran@fcq.unc.edu.ar; lcervi@fcq.unc.edu.ar .1 Dpto. de Bioq. Clín. Fac. C. Qcas. Univ.Nac.Córdoba. CIBICI-CONICET. Córdoba, Argentina. 2 Fac.de Med. Univ. de la República. Uruguay.Fasciola, Cathepsin, dendritic cells, IFN-γCathepsin L3 (CL3) from Fasciola hepatica is highly expressed in the juvenile larvae. CL3 is a cystein protease with collagenolytic activity, however there is no information about its interaction with the immune system. The aim of this work was to study the ability of CL3 to modulate bone marrow-derived dendritic cells (DC) maturation and the capacity of these cells to modify T cell responses. Besides we studied the effect of CL3 on T cell from mice infected with the parasite. DC from mice C57BL/6 mice were differentiated with GM-CSF factor and cultured for 18 h with CL3 produced in Hansenula polymorpha. These cells were then cultured with allogeneic splenocytes. Besides, splenocytes from infected mice were re-stimulated or not with CL3. Cytokine production was detected by ELISA or FACS analysis. CL3 increases the expression of MHC class II in DC, whereas decreases the expression of the costimulatory molecule CD80. Moreover, these cells increase IFN-γ production in cultures with CD8 and CD4 allogeneic T cells (Student test, p<0,05) and this effect was not observed when DC were treated with a mutated CL3. Besides, splenocytes from infected animals re-stimulated with CL3 secrete significant amounts of IFN-γ (Student test, p<0,05).Our results show that CL3-treated DC increase IFN-γ production in allogeneic lymphocytes and this effect it appears to be dependent on the enzymatic activity of CL3. Similarly, CL3 promotes IFN-γ production in splenocytes from infected animals. These data suggest that CL3 would be an interesting target for vaccine development against infection.