Kunitz type molecule-based vaccine elicits elevated specific IgGs and IGA antibodies and Th1/Th17 responses against Fasciola hepatica

SILVANE LEONARDO; CELIAS, DAIANA PAMELA; MALETTO B; FERNANDEZ VALLECILLO F; CHIODETTI AL; ROMAGNOLI P ; MOTRAN CLAUDIA C; PALMA SD; ALLEMANDI DA; . CERVI L. Y MOTRÁN CC

Buenos Aires

Congreso; LXV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI); 2017

SAI

808) KUNITZ TYPE MOLECULE-BASED VACCINE ELICITSELEVATED SPECIFIC IGGS AND IGA ANTIBODIESAND TH1/TH17 RESPONSES AGAINST FASCIOLA HEPATICALeonardo micael Silvane (1), Daiana Celias (1), MalettoBelkys (1), Fernanda Sanchez Vallecillo (1), Ana Chiodetti(1), Romagnoli Pablo (1), Cristina Motran (1), Santiago Palma(2), Daniel Allemandi (2), Laura Cervi (1)(1) Dpto. de Bioq. Clín. Fac. C. Qcas. Univ.Nac.Córdoba. CIBICI-CONICET. Córdoba, Argentina, (2) Dpto de Farmacia,Fac. C. Qcas. Univ. Nac.Córdoba, UNITEFA (CONICET),Córdoba, Argentina.Fasciola hepatica is a helminth parasite with a worldwidedistribution, which can cause a chronic liver disease: fasciolosis,leading to economic losses in the livestock and public health inmany countries. The prevailing control strategy based on anthelminticdrugs is unsustainable due to widespread resistance; hence vaccinationappears as an attractive option to pursue. In our laboratorywe study a vaccine formulated with Kunitz type molecule (KTM), aninhibitor of serine proteases with a key role in the parasite survival,and CpG-ODN/Coa-ASC16, an adjuvant with capacity to induce Th1and Th17 responses.Previous results showed the efficacy of the vaccination in micewhich generates a reduction in the liver damage produced by the infection.The aim of this work was to investigate the immune responsedeveloped during KTM/CpG-ODN/COA-ASC16 immunizationAnimals were allocated in four groups of 4 each. BALB/c micewere subcutaneously inoculated with 10 μg of KTM in CpG-ODN/Coa-ASC16 on days 0-7-14. Control animals were inoculated thesame days with CpG-ODN/Coa-ASC16 or PBS. On day 21, all animalswere orally infected with 6 F. hepatica metacercariae. An additionalgroup without treatment or infection was including as control.After 4 days of challenge the mice were euthanized.Mice vaccinated with KTM/CpG-ODN/Coa-ASC16 showed highlevels of specific IgG1, IgG2a and IgA in plasma (Student test <0,05)compare with another groups. In addition, Increased IgA titers in intestinallavage and feces (student test p<0,05) were observed. Igsdetection were performed by ELISASplenocytes from KTM/CpG-ODN/Coa-ASC16 immunized mice,KTM restimulated, secrete significant amounts of IL-17 and IFN-γ(Student test, p<0,05) by ELISA.These data suggest that the presence of elevated Th1-Th17 mixedresponses and the high levels of IgGs and mucosal IgA antibodies,would be involved in the protection observed by KTM/CpG-ODN/Coa-ASC16 vaccination.Keywords: Fasciola hepatica, Vaccine, Kunitz type molecule,CpG-ODN/Coa-ASC16, IgA