Potent inhibition of Western Equine Encephalitis virus by a fraction rich in flavonoids and phenolic acids obtained from Achyrocline satureioides (Lam) DC.

SABINI C; CARIDDI, LN; ESCOBAR F; MAÑAS F; COMINI LR .; IGLESIAS D; LARRAURI M; NÚÑEZ MONTOYA SC; SERENO J; CONTINGIANI M; CANTERO J; SABINI L

REVISTA BRASILEIRA DE FARMACOGNOSIA

JOHN BENJAMINS PUBLISHING COMPANY

Lugar: Amsterdam; Año: 2016 vol. 26 p. 571 - 571

0102-695X

chyrocline satureioides (Lam.) DC. Asteraceae, ?marcela del campo?, possess several pharmacological properties. Previously we reported antiviral activity of an aqueous extract of A. satureioides against an alphavirus, Western Equine Encephalitis virus. Alphaviruses are highly virulent pathogens which causeencephalitis in humans and equines. There are no effective antiviral to treat its infections. The aim of this study was to evaluate in vitro cytotoxic and antiviral activities against Western Equine Encephalitis virus of five water extract chromatographic fractions from A. satureioides and identify the main compounds of the bioactive fraction. Also, it was to assess in vivo cytogenotoxic ability of the active fraction. Cytotoxicity studies revealed low toxicity of the most of fractions in Vero and in equine peripheral bloodmononuclear cells. Antiviral studies showed that the water crude extract ? Sephadex LH 20 ? fraction 3 MeOH?H2O (Fraction 3) was active against Western Equine Ence