Apoptosis and mutagenicity induction by a characterized aqueous extract of Baccharis articulata

CARIDDI, LN; ESCOBAR F; SABINI C; TORRES C ; REINOSO E; CRISTOFOLINI A ; COMINI L ; NÚÑEZ MONTOYA SC ; SABINI L

FOOD AND CHEMICAL TOXICOLOGY

PERGAMON-ELSEVIER SCIENCE LTD

Año: 2012 vol. 50 p. 155 - 155

0278-6915

n a previous study we have demonstrated that cold aqueous extract of Baccharis articulata (Ba-CAE) induced the death of human peripheral blood mononuclear cells (PBMCs) and exerted low mutagenic effects on mice at 6 h after administration. The aim of this work was to investigate whether the PBMCs death induced by Ba-CAE is due to apoptosis, and whether this extract exerts mutagenic effects on mice at 24 and 48 h after administration. In addition, Ba-CAE was chemically characterized. PBMCs from healthy volunteers were exposed to extract (10, 20, 40, 80, 160, 320, 640 and 1280 lg/mL) for 18?24 h. Cell viability was determined by staining of trypan blue dye exclusion method. Apoptosis was determined by Hoechst 33258 staining, TUNEL, and DNA fragmentation analysis by agarose gel electrophoresis. BALB/c mice were injected with extract (1800, 900 and 450 mg/kg) and sacrificed at 24 and 48 h postinjection. Bone marrow samples were used to assess chromosome mutations by the micronuc