Self-assembled micelles of the (lipo) glycopeptides, teicoplanin, as taxane nanocarriers

COMINI, LAURA RAQUEL; ALASINO, ROXANA VALERIA; LEONHARD, VICTORIA; BIERBRAUER, KARINA LILIAN; BELTRAMO, DANTE MIGUEL

NANOTECHNOLOGY

IOP PUBLISHING LTD

Año: 2021 vol. 32

0957-4484

he use of nanoparticles is one of the strategies currently studied to minimize the toxicity and lack of tissue specificity of many cancer drugs used in chemotherapy. In this research the physicochemical and biological behavior of a novel self-assembled nanostructure of the antibiotic Teicoplanin (Teico) was characterized as a nanocarrier system for solubilizing highly hydrophobic drugs like Paclitaxel (Ptx) in aqueous media. The Teico micelles were loaded with Ptx in DMSO or PEG-400. The interaction between the loaded micelles and Albumin human serum albumin (HSA) was then studied by size exclusion chromatography. Transmission electron microscopy, dynamic light scattering and high-resolution liquid chromatography were also used to characterize the physicochemical and structural properties of the micelles to form the Teico/Ptx and Teico/Ptx/HSA micelles. Cellular uptake of Ptx was evaluated by fluorescent microscopy. The in vitro cytotoxicity of the complexes was studied on Hep-2 tumor