Resumen:
he object of this study is to intensify the process of obtaining of sulfated flavonoids (SFs) from Flaveria bidentis by conventional reflux method. F. bidentis widely distributed in the American Continent is a frequent and highly invasive plant considered weed by farmers and whose weeding generates high vegetal residues. This plant contains SFs in its aerial parts, mostly, 3,7,3´,4´-quercetin tetrasulfate (QTS) and Quercetin 3-acetyl-7,3´,4´-trisulfate (ATS), with important therapeutic potential. In this work, was analyzed the influence of different operation conditions: solvent composition, solvent/sample ratio, time and particle size, on the extraction yield of QTS and ATS. First, solvent composition was selected and later a response surface methodology involving the Doehlert experimental design was applied to determine the effect of the remaining variables. Our results showed that the optimal reflux extraction conditions were 134 mL/g solvent/sample ratio, 0.9 h and a particle siz