Treatment of rats with heat killed cells (HKC) of Cryptococcus neoformans var. grubii induces cellular activation in spleen and lymphatic nodes.

BARONETTI, JL; CHIAPELLO, LS; GARRO, AP; MASIH, DT

ELSEVIER SCI LTD

Año: 2011

p style="TEXT-ALIGN: justify; LINE-HEIGHT: 200%; MARGIN: 0cm 0cm 0pt" class="MsoNormal">Our previous studies showed that the subcutaneous pretreatment of rats with heat killed cells of Cryptococcus neoformans (HKC) emulsified in complete Freund adjuvant (CFA) promotes protection against an intraperitoneal challenge with viable C. neoformans. In this model, an appropriate activation of adherent peritoneal cells after antigenic treatment is very important for the control of the infection. Here, we investigated the immune response developed in spleen and lymphatic nodes as a result of treatment with HKC-CFA, which might also contribute in the protective phenomenon of this treatment against cryptococcal infection. The results show that, compared with adjuvant alone, rats which received treatmen