Early events associated to the oral coadministration of type II collagen and chitosan: induction of anti-inflammatory cytokines

C. PORPORATTO; I.D. BIANCO,; A.M. CABANILLAS; S.G. CORREA

INTERNATIONAL IMMUNOLOGY

Año: 2004 vol. 16 p. 433 - 433

0953-8178

span lang="en" style="FONT-SIZE: 10pt; FONT-FAMILY: Arial; mso-ansi-language: EN-US; mso-fareast-font-family: "Times New Roman"; mso-fareast-language: ES-AR; mso-bidi-language: AR-SA; mso-bidi-font-family: "Times New Roman"">Oral administration of antigens results in systemic hyporesponsiveness termed oral tolerance, largely dependent on the concentration of the relevant antigen in circulation. Strategies to enhance the absorption of intact antigen may be important to improve the tolerance state. Chitosan is a natural polysaccharide able to promote the transmucosal absorption of peptides and proteins. We speculated that the oral tolerance to CII, a frequent target of tolerance studies, could be enhanced by the coadministration of chitosan. We evaluated 16 h after feeding CII and chitosan, molecular events both in mucosal and in systemic lymphoid tissues. We determined in Peyer´s patches (PP) and spleen cells, the activation of T cells, the arrival of the antigens,