Ability of the polysaccharide chitosan to inhibit proliferation of CD4+ lymphocytes from mucosal inductive sites, in vitro and in vivo

PORPORATTO C; CANALI M.M.; BIANCO I.D.; CORREA S.G.

WILEY-BLACKWELL PUBLISHING, INC

Año: 2009 vol. 42 p. 780 - 780

b style="">Abstract. Objective: After oral administration of chitosan, a copolymer of glucosamine and N-acetylglucosamine, mesenteric lymph node (MLN) lymphocytes exhibit traits of anergy, a process coupled with the inability of a mature T cell to proliferate. We wondered whether the biological activity of chitosan could be affecting the division of lymphocytes at the mucosal inductive sites. Materials & methods:  We studied the effect of chitosan on the proliferation of carboxyfluorescein diacetate (CFSE)-labeled MLN lymphocytes stimulated with concanavalin A (ConA) in vitro. We assessed the expression of CD25 and CD71 activation markers and the pro-apoptotic