Selective Binding of Albumin to GM1 Ganglioside Micelles Containing Paclitaxel

V. LEONHARD; ALASINO R.V.; BIANCO I.D.; BELTRAMO D.M.

Journal of Nanomedicine and Nanotechnology

OMICS Publishing Group

Lugar: Los Angeles; Año: 2013 vol. 4 p. 1 - 1

2157-7439

n our previous work we showed that taxanes (paclitaxel (Ptx) and docetaxel (Dtx)) can be spontaneously loaded into ganglioside GM1 nanomicelles, increasing their water solubility about 6,000 times, to render stable water soluble formulations that could be used as a novel strategy to deliver drugs in cancer treatments (Leonhard et al., 2012). Here, we describe the hydrophobic interaction of GM1/Ptx micelles with human serum albumin (HSA), a carrier molecule that could improve tumor drug accumulation. This interaction is regulated by conditions such pH and temperature and generates ternary complexes GM1/Ptx/HSA with sizes around 19 to 24 nm and hydrodynamic radius equivalent to a globular protein of 140-180 kDa. These mixed micelles were stable in solution for at least 40 days and also upon freeze-thawing or lyophilization-solubilization cycles. The results of in vitro assays show that the nano-structures developed are taken up by cell cultures with an antimitotic activity of Ptx on