Biochemical characterization of the interactions between Doxorubicin and lipidic GM1 micelles with or without Paclitaxel loading.

V. LEONHARD; ALASINO R.V.; BIANCO I.D.; GARRO, A.G.; HEREDIA V.; BELTRAMO D.M.

DOVE MEDICAL PRESS LTD

Lugar: Auckland; Año: 2015 vol. 2015 p. 3377 - 3377

oxorubicin (Dox) is an anthracycline anticancer drug with high water solubility, whose use is limited primarily by significant side effects. In this study it is shown that Dox interacts with GM1 ganglioside micelles primarily through hydrophobic interactions independent of pH and ionic strength. In addition, Dox can be incorporated even into GM1 micelles already containing highly hydrophobic Paclitaxel (Ptx). However, it was not possible to incorporate Ptx into Dox containing GM1 micelles, suggesting that Dox could be occupying a more external position in the micelles. This result is in agreement with a higher hydrolysis of Dox than Ptx when the micelles were incubated at alkaline pH. Loading of Dox into GM1 micelles was observed over a broad range of temperature (4 - 55 ºC). Furthermore, Dox-loaded micelles were stable in aqueous solutions exhibiting no aggregation or precipitation for up to 2 months when kept at 4 - 25 ºC and even after freeze-thawing cycles. Upon exposure to bl