Treatment of rats with heat killed cells (HKC) of Cryptococcus neoformans var. grubii induces cellular activation in spleen and lymphatic nodes

BARONETTI JL, CHIAPELLO LS, GARRO AP, MASIH DT

ELSEVIER SCI LTD

Año: 2011 p. 1 - 1

font size="1"> Our previousstudiesshowedthatthesubcutaneouspretreatmentofratswithheatkilled cells of Cryptococcus neoformans (HKC) emulsifiedincompleteFreundadjuvant(CFA)pro- motes protectionagainstanintraperitonealchallengewithviable C. neoformans. Inthis model, anappropriateactivationofadherentperitonealcellsafterantigenictreatmentis very importantforthecontroloftheinfection.Here,weinvestigatedtheimmuneresponse developed inspleenandlymphaticnodesasaresultoftreatmentwithHKC-CFA,which might alsocontributeintheprotectivephenomenonofthistreatmentagainstcryptococ- cal infection.Theresultsshowthat,comparedwithadjuvantalone,ratswhichreceived treatment withHKC-CFApresentedagreateractivationofadherentspleniccells,with up-regulation ofmajorhistocompatibilitycomplexclassII(MHCII)andCD86expression and secretionofanticryptococcalmeta