Oxidative Imbalance in Candida tropicalis Biofilms and Its Relation With Persister Cells

BARONETTI, JOSÉ L.

Frontiers in Microbiology

Lausanne

Año: 2021 vol. 11

ackground: Persister cells (PCs) make up a small fraction of microbial population, cansurvive lethal concentrations of antimicrobial agents. In recent years, Candida tropicalishas emerged as being a frequent fungal agent of medical devices subject to biofilminfections. However, PCs are still poorly understood.Objectives: This study aimed to investigate the relation of PCs on the redox status inC. tropicalis biofilms exposed to high doses of Amphotericin B (AmB), and alterations insurface topography and the architecture of biofilms.Methods: We used an experimental model of two different C. tropicalis biofilmsexposed to AmB at supra minimum inhibitory concentration (SMIC80), and the intra- andextracellular reactive oxygen species (iROS and eROS), reactive nitrogen species (RNS)and oxidative stress response were studied. Light microscopy (LM) and confocal laserscanning microscopy (CLSM) were also used in conjunction with the image analysissoftware COMSTAT.Results: We demonstrated that bi