Resumen:
his study demonstrates the capacity of the onestep
polymerase chain reaction (PCR) fingerprinting method
using the microsatellite primers (GACA)4 or (GTG)5 (MSPPCR)
to identify six of the most frequent dermatophyte species
causing cutaneous mycosis. PCR with (GACA)4 was a
suitable method to recognise Microsporum canis, Microsporum
gypseum, Trichophyton rubrum and Trichophyton interdigitale
among 82 Argentinian clinical isolates, producing the
most simple and reproducible band profiles. In contrast, the
identification of Trichophyton mentagrophytes and Trichophyton
tonsurans was achieved using PCR with (GTG)5. In
this way, the sequential application of PCR using (GACA)4
and (GTG)5 allowed the successful typification of clinical
isolates which had not been determined by mycological standard
techniques. In this work, the intraspecies variability
among 33 clinical isolates of M. canis was detected using
random amplification of polymorphic DNA (RAPD-PCR)
with the primers O