Fasciola hepatica-Induced Immune Suppression of Spleen Mononuclear Cells Proliferation. Role of Nitric Oxide.

. CERVI L, ROSSI GR, CEJAS H AND MASIH DT.

CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY

Año: 1998 vol. 87 p. 145 - 145

0090-1229

p class="MsoNormal" style="MARGIN: 0cm 0cm 10pt">The aim of the present study was to evaluate the proliferative response of spleen mononuclear cells (Spm) to mitogens in rats infected with Fasciola hepatica and its correlation with Spm and peritoneal cell (PC) nitric oxide (NO) production on Days 1, 3, 7, 14, 30, and 60 postinfection. In addition, histological changes in the liver were also studied. The proliferative response to Con A of F. hepatica-infected Spm was significantly decreased on Day 7 postinfection (P < 0.01). However, a pronounced increase of the proliferative response was detected from Day 3 until Day 60 when Spm were stimulated with LPS. In order to determine whether NO levels were modified during F. hepatica infections, we quantified nitrite in Spm and PC supernatants in cultures. Our results indicate a profound decrease of nitrite production by LPS-stimulated PC on the first and second weeks po