Lack of Involvement of Nitric Oxide in the Macrophage-Mediated Inhibition of Spleen Cells Proliferation During Experimental Cryptococcosis.

ROSSI, GR., CERVI, L, SASTRE D. AND T. MASIH, D.

CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY

Elsevier

Año: 1998 vol. 86 p. 16 - 16

0090-1229

p class="MsoNormal" style="MARGIN: 0cm 0cm 10pt">We investigated the proliferative response to mitogens of spleen mononuclear (Spm) cells from Cryptococcus neoformans-infected rats. We determined reactive oxygen intermediates (ROI) and nitric oxide (NO) production by peritoneal and Spm cells, and evaluated the correlation of the proliferative response with NO and ROI production. The proliferative response of Spm cells from infected rats dramatically decreased at 14 and 21 days postinfection (PI). The unresponsiveness of Spm cells from 14-day infected rats was not abrogated by the addition of L-NAME and AG, indicating that NO is not involved in the antiproliferative response of experimental cells. When SOD, catalase, and indomethacin were added to the cultures, the suppression was still observed, indicating that ROI and prostaglandins are not involved in the unresponsiveness of lymphocytes. The proliferative response