Inhibition of spleen cell proliferative response to mitogens by excretory-secretory antigens of Fasciola hepatica.

CERVI L AND MASIH DT.

INTERNATIONAL JOURNAL FOR PARASITOLOGY

Elsevier

Año: 1997 vol. 27 p. 573 - 573

0020-7519

p class="MsoNormal" style="MARGIN: 0cm 0cm 10pt">The effect of Fasciola hepatica excretory-secretory antigen (ESA) on the proliferative response of spleen mononuclear (SpM) cells of normal rats to stimulation with mitogens has been examined. When ESA was added to normal SpM cells, there was a decrease in the proliferative response to concanavalin A (Con A) or lipo-polysaccharide (LPS) in a dose-dependent manner. The addition of indomethacin, which blocks prostaglandin synthesis, or N omega-nitro-L-arginine methyl ester (L-NAME) a specific inhibitor of nitric oxide (NO) synthase, had no effect on the ability of ESA to suppress the proliferative response to Con A. However, supplementation of the culture media with catalase, which degrades hydrogen peroxide (H2O2) or superoxide dismutase (SOD) to remove superoxide anion (O2), resulted in a restoration of proliferation to Con A. When LPS was used as mitogenic stimulus n