Involvement of a mitochondrial pathway and key role of hydrogen peroxide during eosinophil apoptosis induced by excretory-secretory products from Fasciola hepatica

SERRADELL MC, GUASCONI L, MASIH DT.

Elsevier

Lugar: Amsterdam; Año: 2009 vol. 163 p. 95 - 95

font size="1" face="OneGulliverA"> Eosinophils (Eo) are typically associated with immune response to helminth. Previously, we demonstrated that excretory–secretory products (ESP) from Fasciola hepatica induce eosinophil apoptosis by a caspase-dependent mechanism. In this study, we observed that ESP caused mitochondrial-membrane depolarization of eosinophils leading to the release of cytochrome c. Also, ESP induced an increase in the reactive oxygen species (ROS) production, which preceded the mitochondrial injury. We found a significant rise in hydrogen peroxide, but not in the anion superoxide levels. Furthermore, catalase, but not superoxide dismutase, inhibited the mitochondrial depolarization as well as apoptosis. So, ESP induce in E