Serological, electrophoretic and biological properties of Fasciola hepatica antigens.

CERVI LA, RUBINSTEIN H, MASIH DT

Revista del Institulo de Medicina tropical de Sao Paulo

Lugar: San Pablo, Brasil; Año: 1992 vol. 34 p. 517 - 517

p class="MsoNormal" style="MARGIN: 12pt 0cm 0pt 42pt; LINE-HEIGHT: 14.4pt">Fasciola hepatica somatic antigen, its partially purified fractions and excretion-secretion products were investigated as to serological, electrophoretic and biological properties. In a Sephadex G-100 column (SG-100), Fasciola hepatica total antigen (FhTA) gave 5 fractions, and SDS-PAGE analysis showed they were glycoproteins ranging from 14 to 94 kDa molecular weight (MW). When these fractions were analyzed by enzyme-linked immunotransfer blot (EITB) and immunodiffusion in gel (ID) with serum from immunized rats with FhTA, the presence of different antigenic components was revealed. In the SDS-PAGE of excretor-secretor antigen (ESA), it was possible to observe peptides from 12 to 22 kDa, which were also present in FhTA. When the FhTA, its fractions and the ESA were analyzed by EITB with the immune rat serum (IRS), it was obs