Polyphenol analysis in human milk by a rapid, cost-effective, and accurate micromethod: translational development

CORTEZ, MARIELA VALENTINA; SCOTTA, ANA VERÓNICA; MIRANDA, AGUSTIN RAMIRO; SORIA, ELIO ANDRES

REVISTA PAULISTA DE PEDIATRIA

Elsevier

Lugar: Sao Paulo; Año: 2024

0103-0582

UMMARYObjective: To develop a rapid method for analysing polyphenols, which are potentially active antioxidants against neonatal oxidative stress, from small human milk (HM) volumes.Methods: Acid and alkaline extractions were compared using two dyes: Folin-Ciocalteu and Fast Blue BB. Linearity, sensitivity, recovery percentage, polyphenol content, precision, and stability were assessed in 14 HM samples and compared using the Kruskal-Wallis H test (p0.96) up to 500 mg/L. All Bicarbonate-based Folin-Ciocalteu methods assayed were stable and repeatable, whereas Fast Blue BB-based variants were not. HM polyphenols (mean=94.68 mg/L) positive