Buscador de Personas - SIGEVA - Universidad Nacional de Córdoba

Chlorpyrifos is a pesticide with widely used in agriculture, and its exposure is linked to several human pathologies, such as neurological disorders, cancer and immunosuppression. The use of phytoextracts has been proposed to control this toxicity by their bioactive phytochemical content. Thus, our aim was to identify protective extracts (doses: 0-100 ug/mL) derived from infusions of Argentinean plants in cultures of murine splenocytes and lymphocytes exposed to chlorpyrifos (doses: 0-35 ug/mL). Cellular viability (CV), γ-glutamyltranspeptidase activity(GGT), aqueous and lipid hidroperoxides (AHP and LHP) and nitrites (N) were measured. A significant toxicity was found in splenocytes (total non-activated lymphocytes and other splenic immunocytes) with 35 ug/mL chlorpyrifos, which reduced CV to 61±9.5%. This was associated with N reduction (17±1.7%) and GGT induction (267±33.3%), with the consequent AHP decrease (57±3.8%), without LHP changes (108±15.0%). The infusive extracts of Aspidosperma quebrachoblanco,Ilex paraguariensis and Mandevila pentladiana prevented splenocyte death treatedwith 35 ug/mL chlorpyrifos. When mitogen-activated lymphocytes were studied, Bcells tolerated toxic exposure better than T cells (p<0.05). Only M. pentladiana maintained CV in B lymphocytes, although the three phytoextracts were antioxidant. None of the phytoextracts test showed lymphoprotective capacity in T cells. We conclude that proliferating cells were more sensitive to chlorpyrifos, and among them T lymphocytes had less resistance. Thus the studied extracts protected splenocytes but not lymphocytes at the assayed doses.

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