On-line Biomass Monitoring in Bench-Scale Stirred Bioreactors Using Parts of a Chromatography System

SPRINGER

Lugar: Londres; Año: 2002 vol. 24 p. 1999 - 1999

cell density monitor, comprising parts of a liquid chromatography system, was adapted for on-line real-time measurement of bacterial cell density up to 75 g l?1. The device dilutes the sample containing cells and measures the absorbancy at 280 nm. This detector gives an excellent correlation with off-line measurements, good reproducibility, a low limit of detection (3.5 mg l?1), and becomes a low cost alternative for fermentation monitoring in small stirred bioreactors.